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JCIMPT Publications

Publications - 2014

93. H. Wu, C. Wang, K. J. Gregory, G. W. Han, H. P. Cho, Y. Xia, C. M. Niswender, V. Katritch, J. Meiler, V. Cherezov, P. J. Conn and R. C. Stevens (2014) Structure of a class C GPCR metabotropic glutamate receptor 1 bound to an allosteric modulator Science 344: 58-64 ;
92. U. Weierstall, D. James, C. Wang, T. A. White, D. Wang, W. Liu, J. C. Spence, R. Bruce Doak, G. Nelson, P. Fromme, R. Fromme, I. Grotjohann, C. Kupitz, N. A. Zatsepin, H. Liu, S. Basu, D. Wacker, G. W. Han, V. Katritch, S. Boutet, M. Messerschmidt, G. J. Williams, J. E. Koglin, M. Marvin Seibert, M. Klinker, C. Gati, R. L. Shoeman, A. Barty, H. N. Chapman, R. A. Kirian, K. R. Beyerlein, R. C. Stevens, D. Li, S. T. Shah, N. Howe, M. Caffrey and V. Cherezov (2014) Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography Nat Commun 5: 3309 ;
91. C. Wang, H. Wu, T. Evron, E. Vardy, G. W. Han, X. P. Huang, S. J. Hufeisen, T. J. Mangano, D. J. Urban, V. Katritch, V. Cherezov, M. G. Caron, B. L. Roth and R. C. Stevens (2014) Structural basis for Smoothened receptor modulation and chemoresistance to anticancer drugs Nat Commun 5: 4355 ;
90. L. Susac, R. Horst and K. Wuthrich (2014) Solution-NMR characterization of outer-membrane protein A from E. coli in lipid bilayer nanodiscs and detergent micelles Chembiochem 15: 995-1000 ;
89. A. A. Skjevik, M. Mileni, A. Baumann, O. Halskau, K. Teigen, R. C. Stevens and A. Martinez (2014) The N-terminal sequence of tyrosine hydroxylase is a conformationally versatile motif that binds 14-3-3 proteins and membranes J Mol Biol 426: 150-68 ;
88. W. Liu, D. Wacker, C. Wang, E. Abola and V. Cherezov (2014) Femtosecond crystallography of membrane proteins in the lipidic cubic phase Philos Trans R Soc Lond B Biol Sci 369:
87. G. Fenalti, P. M. Giguere, V. Katritch, X. P. Huang, A. A. Thompson, V. Cherezov, B. L. Roth and R. C. Stevens (2014) Molecular control of delta-opioid receptor signalling Nature 506: 191-6 ;

Publications - 2013

86. C. Wang, H. Wu, V. Katritch, G. W. Han, X. P. Huang, W. Liu, F. Y. Siu, B. L. Roth, V. Cherezov and R. C. Stevens (2013) Structure of the human smoothened receptor bound to an antitumour agent Nature 497: 338-43 ;
85. C. Wang, Y. Jiang, J. Ma, H. Wu, D. Wacker, V. Katritch, G. W. Han, W. Liu, X. P. Huang, E. Vardy, J. D. McCorvy, X. Gao, X. E. Zhou, K. Melcher, C. Zhang, F. Bai, H. Yang, L. Yang, H. Jiang, B. L. Roth, V. Cherezov, R. C. Stevens and H. E. Xu (2013) Structural basis for molecular recognition at serotonin receptors Science 340: 610-4 ;
84. D. Wacker, C. Wang, V. Katritch, G. W. Han, X. P. Huang, E. Vardy, J. D. McCorvy, Y. Jiang, M. Chu, F. Y. Siu, W. Liu, H. E. Xu, V. Cherezov, B. L. Roth and R. C. Stevens (2013) Structural features for functional selectivity at serotonin receptors Science 340: 615-9 ;
83. H. Tao, S. C. Lee, A. Moeller, R. S. Roy, F. Y. Siu, J. Zimmermann, R. C. Stevens, C. S. Potter, B. Carragher and Q. Zhang (2013) Engineered nanostructured beta-sheet peptides protect membrane proteins Nat Methods 10: 759-61 ;
82. F. Y. Siu, M. He, C. de Graaf, G. W. Han, D. Yang, Z. Zhang, C. Zhou, Q. Xu, D. Wacker, J. S. Joseph, W. Liu, J. Lau, V. Cherezov, V. Katritch, M. W. Wang and R. C. Stevens (2013) Structure of the human glucagon class B G-protein-coupled receptor Nature 499: 444-9 ;
81. S. A. Seidel, P. M. Dijkman, W. A. Lea, G. van den Bogaart, M. Jerabek-Willemsen, A. Lazic, J. S. Joseph, P. Srinivasan, P. Baaske, A. Simeonov, I. Katritch, F. A. Melo, J. E. Ladbury, G. Schreiber, A. Watts, D. Braun and S. Duhr (2013) Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions Methods 59: 301-15 ;
80. J. T. Madden, S. J. Toth, C. M. Dettmar, J. A. Newman, R. A. Oglesbee, H. G. Hedderich, R. M. Everly, M. Becker, J. A. Ronau, S. K. Buchanan, V. Cherezov, M. E. Morrow, S. Xu, D. Ferguson, O. Makarov, C. Das, R. Fischetti and G. J. Simpson (2013) Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline J Synchrotron Radiat 20: 531-40 ;
79. W. Liu, D. Wacker, C. Gati, G. W. Han, D. James, D. Wang, G. Nelson, U. Weierstall, V. Katritch, A. Barty, N. A. Zatsepin, D. Li, M. Messerschmidt, S. Boutet, G. J. Williams, J. E. Koglin, M. M. Seibert, C. Wang, S. T. Shah, S. Basu, R. Fromme, C. Kupitz, K. N. Rendek, I. Grotjohann, P. Fromme, R. A. Kirian, K. R. Beyerlein, T. A. White, H. N. Chapman, M. Caffrey, J. C. Spence, R. C. Stevens and V. Cherezov (2013) Serial femtosecond crystallography of G protein-coupled receptors Science 342: 1521-4 ;
78. S. C. Lee, B. C. Bennett, W. X. Hong, Y. Fu, K. A. Baker, J. Marcoux, C. V. Robinson, A. B. Ward, J. R. Halpert, R. C. Stevens, C. D. Stout, M. J. Yeager and Q. Zhang (2013) Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins Proc Natl Acad Sci U S A 110: E1203-11 ;
77. D. J. Kissick, C. M. Dettmar, M. Becker, A. M. Mulichak, V. Cherezov, S. L. Ginell, K. P. Battaile, L. J. Keefe, R. F. Fischetti and G. J. Simpson (2013) Towards protein-crystal centering using second-harmonic generation (SHG) microscopy Acta Crystallogr D Biol Crystallogr 69: 843-51 ;
76. V. Katritch, V. Cherezov and R. C. Stevens (2013) Structure-Function of the G Protein-Coupled Receptor Superfamily Annu Rev Pharmacol Toxicol 53: 531-556 ;
75. T. Didenko, J. J. Liu, R. Horst, R. C. Stevens and K. Wuthrich (2013) Fluorine-19 NMR of integral membrane proteins illustrated with studies of GPCRs Curr Opin Struct Biol 23: 740-7 ;

Publications - 2012

74. Y. Xu, A. Moeller, S. Y. Jun, M. Le, B. Y. Yoon, J. S. Kim, K. Lee and N. C. Ha (2012) Assembly and channel opening of outer membrane protein in tripartite drug efflux pumps of Gram-negative bacteria J Biol Chem 287: 11740-50 ;
73. H. Wu, D. Wacker, M. Mileni, V. Katritch, G. W. Han, E. Vardy, W. Liu, A. A. Thompson, X. P. Huang, F. I. Carroll, S. W. Mascarella, R. B. Westkaemper, P. D. Mosier, B. L. Roth, V. Cherezov and R. C. Stevens (2012) Structure of the human kappa-opioid receptor in complex with JDTic Nature 485: 327-332 ;
72. P. R. Wilderman, S. C. Gay, H. H. Jang, Q. Zhang, C. D. Stout and J. R. Halpert (2012) Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active-site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme FEBS J 279: 1607-20 ;
71. J. F. White, N. Noinaj, Y. Shibata, J. Love, B. Kloss, F. Xu, J. Gvozdenovic-Jeremic, P. Shah, J. Shiloach, C. G. Tate and R. Grisshammer (2012) Structure of the agonist-bound neurotensin receptor Nature 490: 508-13 ;
70. A. A. Thompson, W. Liu, E. Chun, V. Katritch, H. Wu, E. Vardy, X. P. Huang, C. Trapella, R. Guerrini, G. Calo, B. L. Roth, V. Cherezov and R. C. Stevens (2012) Structure of the nociceptin/orphanin FQ receptor in complex with a peptide mimetic Nature 485: 395-9 ;
69. H. Tao, Y. Fu, A. A. Thompson, S. C. Lee, N. Mahoney, R. C. Stevens and Q. Zhang (2012) Synthesis and Properties of Dodecyl Trehaloside Detergents for Membrane Protein Studies Langmuir 28: 11173-81. ;
68. P. Stanczak, Q. Zhang, R. Horst, P. Serrano and K. Wuthrich (2012) Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles J Biomol NMR 54: 129-133 ;
67. J. S. Santos, G. A. Asmar-Rovira, G. W. Han, W. Liu, R. Syeda, V. Cherezov, K. A. Baker, R. C. Stevens and M. Montal (2012) Crystal Structure of a Voltage-gated K+ Channel Pore Module in a Closed State in Lipid Membranes J Biol Chem 287: 43063-70 ;
66. A. Moeller, C. Zhao, M. G. Fried, E. M. Wilson-Kubalek, B. Carragher and S. W. Whiteheart (2012) Nucleotide-dependent conformational changes in the N-Ethylmaleimide Sensitive Factor (NSF) and their potential role in SNARE complex disassembly J Struct Biol 177: 335-43 ;
65. A. Moeller, R. N. Kirchdoerfer, C. S. Potter, B. Carragher and I. A. Wilson (2012) Organization of the Influenza Virus Replication Machinery Science 338: 1631-1634 ;
64. W. Liu, E. Chun, A. A. Thompson, P. Chubukov, F. Xu, V. Katritch, G. W. Han, C. B. Roth, L. H. Heitman, I. J. AP, V. Cherezov and R. C. Stevens (2012) Structural basis for allosteric regulation of GPCRs by sodium ions Science 337: 232-6 ;
63. J. J. Liu, R. Horst, V. Katritch, R. C. Stevens and K. Wuthrich (2012) Biased signaling pathways in beta2-adrenergic receptor characterized by 19F-NMR Science 335: 1106-10 ;
62. X. Jiang, L. Guan, Y. Zhou, W. X. Hong, Q. Zhang and H. R. Kaback (2012) Evidence for an intermediate conformational state of LacY Proc Natl Acad Sci U S A 109: E698-704 ;
61. R. Horst, P. Stanczak, P. Serrano and K. Wuthrich (2012) Translational Diffusion Measurements by Microcoil NMR in Aqueous Solutions of the Fos-10 Detergent-Solubilized Membrane Protein OmpX J Phys Chem B 116: 6775-80 ;
60. M. A. Hanson, C. B. Roth, E. Jo, M. T. Griffith, F. L. Scott, G. Reinhart, H. Desale, B. Clemons, S. M. Cahalan, S. C. Schuerer, M. G. Sanna, G. W. Han, P. Kuhn, H. Rosen and R. C. Stevens (2012) Crystal structure of a lipid G protein-coupled receptor Science 335: 851-5 ;
59. J. W. Fairman, N. Dautin, D. Wojtowicz, W. Liu, N. Noinaj, T. J. Barnard, E. Udho, T. M. Przytycka, V. Cherezov and S. K. Buchanan (2012) Crystal Structures of the Outer Membrane Domain of Intimin and Invasin from Enterohemorrhagic E. coli and Enteropathogenic Y. pseudotuberculosis Structure 20: 1233-43 ;
58. E. Chun, A. A. Thompson, W. Liu, C. B. Roth, M. T. Griffith, V. Katritch, J. Kunken, F. Xu, V. Cherezov, M. A. Hanson and R. C. Stevens (2012) Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors Structure 20: 967-76 ;
57. Author: E. E. Abola, V. Cherezov and R. C. Stevens Year: 2012 Title: X-ray crystallography: crystallization Secondary Author: E. H. Egelman Secondary Title: Comprehensive Biophysics Place Published: Oxford Publisher: Academic Press Volume: 1 Pages: 34-63 Tertiary Author: H. J. Dyson Tertiary Title: Biophysical Techniques for Structural Characterization of Macromolecules Short Title: X-ray crystallography: crystallization

Publications - 2011

56. Q. Zhang, H. Tao and W. X. Hong (2011) New amphiphiles for membrane protein structural biology Methods 55: 318-23 ;
55. Y. Xu, S. Song, A. Moeller, N. Kim, S. Piao, S. H. Sim, M. Kang, W. Yu, H. S. Cho, I. Chang, K. Lee and N. C. Ha (2011) Functional implications of an intermeshing cogwheel-like interaction between TolC and MacA in the action of macrolide-specific efflux pump MacAB-TolC J Biol Chem 286: 13541-9 ;
54. Y. Xu, M. Lee, A. Moeller, S. Song, B. Y. Yoon, H. M. Kim, S. Y. Jun, K. Lee and N. C. Ha (2011) Funnel-like Hexameric Assembly of the Periplasmic Adapter Protein in the Tripartite Multidrug Efflux Pump in Gram-negative Bacteria J Biol Chem 286: 17910-20 ;
53. F. Xu, H. Wu, V. Katritch, G. W. Han, K. A. Jacobson, Z. G. Gao, V. Cherezov and R. C. Stevens (2011) Structure of an agonist-bound human A2A adenosine receptor Science 332: 322-7 ;
52. F. Xu, W. Liu, M. A. Hanson, R. C. Stevens and V. Cherezov (2011) Development of an Automated High Throughput LCP-FRAP Assay to Guide Membrane Protein Crystallization in Lipid Mesophases Cryst Growth Des 11: 1193-1201 ;
51. T. Tiefenbrunn, W. Liu, Y. Chen, V. Katritch, C. D. Stout, J. A. Fee and V. Cherezov (2011) High resolution structure of the ba3 cytochrome c oxidase from Thermus thermophilus in a lipidic environment PLoS One 6: e22348 ;
50. A. A. Thompson, J. J. Liu, E. Chun, D. Wacker, H. Wu, V. Cherezov and R. C. Stevens (2011) GPCR stabilization using the bicelle-like architecture of mixed sterol-detergent micelles Methods 55: 310-7 ;
49. T. Shimamura, M. Shiroishi, S. Weyand, H. Tsujimoto, G. Winter, V. Katritch, R. Abagyan, V. Cherezov, W. Liu, G. W. Han, T. Kobayashi, R. C. Stevens and S. Iwata (2011) Structure of the human histamine H(1) receptor complex with doxepin Nature 475: 65-70 ;
48. M. B. Shah, J. Pascual, Q. Zhang, C. D. Stout and J. R. Halpert (2011) Structures of cytochrome P450 2B6 bound to 4-benzylpyridine and 4-(4-nitrobenzyl)pyridine: insight into inhibitor binding and rearrangement of active site side chains Mol Pharmacol 80: 1047-55 ;
47. M. T. Miller, M. Mileni, D. Comoletti, R. C. Stevens, M. Harel and P. Taylor (2011) The Crystal Structure of the alpha-Neurexin-1 Extracellular Region Reveals a Hinge Point for Mediating Synaptic Adhesion and Function Structure 19: 767-78 ;
46. A. C. Milazzo, A. Cheng, A. Moeller, D. Lyumkis, E. Jacovetty, J. Polukas, M. H. Ellisman, N. H. Xuong, B. Carragher and C. S. Potter (2011) Initial evaluation of a direct detection device detector for single particle cryo-electron microscopy J Struct Biol 176: 404-8 ;
45. W. Liu and V. Cherezov (2011) Crystallization of membrane proteins in lipidic mesophases J Vis Exp
44. J. S. Joseph, W. Liu, J. Kunken, T. M. Weiss, H. Tsuruta and V. Cherezov (2011) Characterization of lipid matrices for membrane protein crystallization by high-throughput small angle X-ray scattering Methods 55: 342-9 ;
43. R. Horst, A. L. Horwich and K. Wuthrich (2011) Translational diffusion of macromolecular assemblies measured using transverse-relaxation-optimized pulsed field gradient NMR J Am Chem Soc 133: 16354-7 ;
42. L. M. Haupert and G. J. Simpson (2011) Screening of protein crystallization trials by second order nonlinear optical imaging of chiral crystals (SONICC) Methods 55: 379-86 ;
41. S. C. Gay, H. Zhang, P. R. Wilderman, A. G. Roberts, T. Liu, S. Li, H. L. Lin, Q. Zhang, V. L. Woods, Jr., C. D. Stout, P. F. Hollenberg and J. R. Halpert (2011) Structural analysis of mammalian cytochrome P450 2B4 covalently bound to the mechanism-based inactivator tert-butylphenylacetylene: insight into partial enzymatic activity Biochemistry 50: 4903-11 ;
40. L. S. Fisher, A. Ward, R. A. Milligan, N. Unwin, C. S. Potter and B. Carragher (2011) A helical processing pipeline for EM structure determination of membrane proteins Methods 55: 350-62 ;
39. V. Cherezov (2011) Lipidic cubic phase technologies for membrane protein structural studies Curr Opin Struct Biol 21: 559-66 ;
38. M. M. Baksh, A. K. Kussrow, M. Mileni, M. G. Finn and D. J. Bornhop (2011) Label-free quantification of membrane-ligand interactions using backscattering interferometry Nat Biotechnol 29: 357-60 ;

Publications - 2010

37. X. Zhang, E. Y. Chien, M. J. Chalmers, B. D. Pascal, J. Gatchalian, R. C. Stevens and P. R. Griffin (2010) Dynamics of the beta2-adrenergic G-protein coupled receptor revealed by hydrogen-deuterium exchange Anal Chem 82: 1100-8 ;
36. B. Wu, E. Y. Chien, C. D. Mol, G. Fenalti, W. Liu, V. Katritch, R. Abagyan, A. Brooun, P. Wells, F. C. Bi, D. J. Hamel, P. Kuhn, T. M. Handel, V. Cherezov and R. C. Stevens (2010) Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists Science 330: 1066-71 ;
35. D. Wacker, G. Fenalti, M. A. Brown, V. Katritch, R. Abagyan, V. Cherezov and R. C. Stevens (2010) Conserved binding mode of human beta2 adrenergic receptor inverse agonists and antagonist revealed by X-ray crystallography J Am Chem Soc 132: 11443-5 ;
34. T. A. Nguyen, S. S. Lieu and G. Chang (2010) An Escherichia coli-based cell-free system for large-scale production of functional mammalian membrane proteins suitable for X-ray crystallography J Mol Microbiol Biotechnol 18: 85-91 ;
33. M. Michino, J. Chen, R. C. Stevens and C. L. Brooks, 3rd (2010) FoldGPCR: structure prediction protocol for the transmembrane domain of G protein-coupled receptors from class A Proteins 78: 2189-201 ;
32. W. Liu, M. A. Hanson, R. C. Stevens and V. Cherezov (2010) LCP-Tm: an assay to measure and understand stability of membrane proteins in a membrane environment Biophys J 98: 1539-48 ;
31. D. J. Kissick, E. J. Gualtieri, G. J. Simpson and V. Cherezov (2010) Nonlinear optical imaging of integral membrane protein crystals in lipidic mesophases Anal Chem 82: 491-7 ;
30. V. Katritch, V. P. Jaakola, J. R. Lane, J. Lin, A. P. Ijzerman, M. Yeager, I. Kufareva, R. C. Stevens and R. Abagyan (2010) Structure-Based Discovery of Novel Chemotypes for Adenosine A(2A) Receptor Antagonists J Med Chem 53: 1799-1809 ;
29. V. P. Jaakola, J. R. Lane, J. Y. Lin, V. Katritch, A. P. Ijzerman and R. C. Stevens (2010) Identification and characterization of amino acid residues essential for human A2A adenosine receptor : ZM241385 binding and subtype selectivity J Biol Chem 285: 13032-13044 ;
28. W. X. Hong, K. A. Baker, X. Ma, R. C. Stevens, M. Yeager and Q. Zhang (2010) Design, synthesis, and properties of branch-chained maltoside detergents for stabilization and crystallization of integral membrane proteins: human connexin 26 Langmuir 26: 8690-6 ;
27. X. He, P. Szewczyk, A. Karyakin, M. Evin, W. X. Hong, Q. Zhang and G. Chang (2010) Structure of a cation-bound multidrug and toxic compound extrusion transporter Nature 467: 991-4 ;
26. S. C. Gay, M. B. Shah, J. C. Talakad, K. Maekawa, A. G. Roberts, P. R. Wilderman, L. Sun, J. Y. Yang, S. C. Huelga, W. X. Hong, Q. Zhang, C. D. Stout and J. R. Halpert (2010) Crystal structure of a cytochrome P450 2B6 genetic variant in complex with the inhibitor 4-(4-chlorophenyl)imidazole at 2.0-A resolution Mol Pharmacol 77: 529-38 ;
25. S. C. Gay, A. G. Roberts, K. Maekawa, J. C. Talakad, W. X. Hong, Q. Zhang, C. D. Stout and J. R. Halpert (2010) Structures of cytochrome P450 2B4 complexed with the antiplatelet drugs ticlopidine and clopidogrel Biochemistry 49: 8709-20 ;
24. Y. Fu, Y. Weng, W. X. Hong and Q. Zhang (2010) Efficient Synthesis of Unsaturated 1-Monoacyl Glycerols for in meso Crystallization of Membrane Proteins Synlett 2011: 809-812 ;
23. E. Y. Chien, W. Liu, Q. Zhao, V. Katritch, G. W. Han, M. A. Hanson, L. Shi, A. H. Newman, J. A. Javitch, V. Cherezov and R. C. Stevens (2010) Structure of the human dopamine D3 receptor in complex with a D2/D3 selective antagonist Science 330: 1091-5 ;

Publications - 2009

22. P. Stanczak, R. Horst, P. Serrano and K. Wuthrich (2009) NMR characterization of membrane protein-detergent micelle solutions by use of microcoil equipment J Am Chem Soc 131: 18450-6 ;
21. M. Michino, E. Abola, C. L. Brooks, 3rd, J. S. Dixon, J. Moult and R. C. Stevens (2009) Community-wide assessment of GPCR structure modelling and ligand docking: GPCR Dock 2008 Nat Rev Drug Discov 8: 455-63 ;
20. V. Katritch, K. A. Reynolds, V. Cherezov, M. A. Hanson, C. B. Roth, M. Yeager and R. Abagyan (2009) Analysis of full and partial agonists binding to beta2-adrenergic receptor suggests a role of transmembrane helix V in agonist-specific conformational changes J Mol Recognit 22: 307-18 ;
19. M. A. Hanson and R. C. Stevens (2009) Discovery of new GPCR biology: one receptor structure at a time Structure 17: 8-14 ;
18. V. Cherezov, M. A. Hanson, M. T. Griffith, M. C. Hilgart, R. Sanishvili, V. Nagarajan, S. Stepanov, R. F. Fischetti, P. Kuhn and R. C. Stevens (2009) Rastering strategy for screening and centring of microcrystal samples of human membrane proteins with a sub-10 microm size X-ray synchrotron beam J R Soc Interface 6 Suppl 5: S587-97 ;
17. M. Caffrey and V. Cherezov (2009) Crystallizing membrane proteins using lipidic mesophases Nat Protoc 4: 706-31 ;
16. S. G. Aller, J. Yu, A. Ward, Y. Weng, S. Chittaboina, R. Zhuo, P. M. Harrell, Y. T. Trinh, Q. Zhang, I. L. Urbatsch and G. Chang (2009) Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding Science 323: 1718-22 ;

Publications - 2008

15. Q. Zhang, R. Horst, M. Geralt, X. Ma, W. X. Hong, M. G. Finn, R. C. Stevens and K. Wuthrich (2008) Microscale NMR screening of new detergents for membrane protein structural biology J Am Chem Soc 130: 7357-63 ;
14. C. B. Roth, M. A. Hanson and R. C. Stevens (2008) Stabilization of the human beta2-adrenergic receptor TM4-TM3-TM5 helix interface by mutagenesis of Glu122(3.41), a critical residue in GPCR structure J Mol Biol 376: 1305-19 ;
13. J. D. Ng, R. C. Stevens and P. Kuhn (2008) Protein crystallization in restricted geometry: advancing old ideas for modern times in structural proteomics Methods Mol Biol 426: 363-76 ;
12. J. D. Ng, P. J. Clark, R. C. Stevens and P. Kuhn (2008) In situ X-ray analysis of protein crystals in low-birefringent and X-ray transmissive plastic microchannels Acta Crystallogr D Biol Crystallogr 64: 189-97 ;
11. V. P. Jaakola, M. T. Griffith, M. A. Hanson, V. Cherezov, E. Y. Chien, J. R. Lane, A. P. Ijzerman and R. C. Stevens (2008) The 2.6 Angstrom Crystal Structure of a Human A2A Adenosine Receptor Bound to an Antagonist Science
10. M. A. Hanson, V. Cherezov, M. T. Griffith, C. B. Roth, V. P. Jaakola, E. Y. Chien, J. Velasquez, P. Kuhn and R. C. Stevens (2008) A specific cholesterol binding site is established by the 2.8 A structure of the human beta2-adrenergic receptor Structure 16: 897-905 ;
9. A. I. Alexandrov, M. Mileni, E. Y. Chien, M. A. Hanson and R. C. Stevens (2008) Microscale fluorescent thermal stability assay for membrane proteins Structure 16: 351-9 ;
8. E. E. Abola and R. C. Stevens (2008) The importance of target selection strategies in structural biology J. L. Sussman and I. Silman Structural proteomics and its impact on the life sciences Singapore World Scientific Publishing Co. 1-28

Publications - 2007

7. Q. Zhang, X. Ma, A. Ward, W. X. Hong, V. P. Jaakola, R. C. Stevens, M. G. Finn and G. Chang (2007) Designing facial amphiphiles for the stabilization of integral membrane proteins Angew Chem Int Ed Engl 46: 7023-5 ;
6. A. Ward, C. L. Reyes, J. Yu, C. B. Roth and G. Chang (2007) Flexibility in the ABC transporter MsbA: Alternating access with a twist Proc Natl Acad Sci U S A 104: 19005-10 ;
5. D. M. Rosenbaum, V. Cherezov, M. A. Hanson, S. G. Rasmussen, F. S. Thian, T. S. Kobilka, H. J. Choi, X. J. Yao, W. I. Weis, R. C. Stevens and B. K. Kobilka (2007) GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function Science 318: 1266-73 ;
4. M. A. Hanson, A. Brooun, K. A. Baker, V. P. Jaakola, C. Roth, E. Y. Chien, A. Alexandrov, J. Velasquez, L. Davis, M. Griffith, K. Moy, B. K. Ganser-Pornillos, Y. Hua, P. Kuhn, S. Ellis, M. Yeager and R. C. Stevens (2007) Profiling of membrane protein variants in a baculovirus system by coupling cell-surface detection with small-scale parallel expression Protein Expr Purif 56: 85-92 ;
3. V. Cherezov, D. M. Rosenbaum, M. A. Hanson, S. G. Rasmussen, F. S. Thian, T. S. Kobilka, H. J. Choi, P. Kuhn, W. I. Weis, B. K. Kobilka and R. C. Stevens (2007) High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor Science 318: 1258-65 ;
2. Y. J. Chen, O. Pornillos, S. Lieu, C. Ma, A. P. Chen and G. Chang (2007) X-ray structure of EmrE supports dual topology model Proc Natl Acad Sci U S A 104: 18999-9004 ;

Publications - 2005

1. F. Katzen, G. Chang and W. Kudlicki (2005) The past, present and future of cell-free protein synthesis Trends Biotechnol 23: 150-6 ;